MYCOPLASMA DETECTION SERVICES

Affinity Molecules offers Mycoplasma Detection Services for common mycoplasma contamination. Our proprietary detection kit is based on PCR detection of DNA from common mycoplasma strains (over 60 stains). The protocol provides direct reliable detection of mycoplasma contamination in culture medium eliminating any sample preparation steps. The inclusion of internal positive control for PCR reaction as well as positive and negative controls simplifies interpretation of data.

Example of mycoplasma detection experiment. Culture supernatant from a contaminated cell line was added directly to PCR reaction without any manipulations resulting in mycoplasma-specific ~270 bp band (Myco Pos, ND). The same supernatant was serially diluted (ND – non-diluted and 1:10, 1:100 and 1:1K-fold dilutions) and added to a series of PCR reactions in the presence of Internal Control. In the absence of mycoplasma contamination, Internal Control is amplified as ~400 bp band. Upon increasing concentrations of mycoplasma DNA, Internal Control band is amplified less efficiently and generation of mycoplasma-specific ~270 bp band takes place. Neg Ctrl – PCR reaction without Internal Control and mycoplasma contamination. Nsp band – a ~140 bp fragment amplified in the presence of Internal Control.