BIOLOGICAL ASSAY DEVELOPMENT

Affinity Molecules offers development of various biological assays. The assays encompass areas of protein quantification and biological activities.

• ELISA-based assays are available in a variety of formats depending on the nature of analytes, availability of detection reagents and the scientific scope. A robust validation procedure enables easy assay transfer process.
• Development and optimization of assays based on flow cytometry use. The approaches include utilization of live or fixated cells and various size beads for ligand presentation.
• Plasmon resonance is a ‘gold standard’ for quantification of inter-molecule interactions. We offer various ligand immobilization options and perform binding and kinetic experiments.
• Cell-based assays are often the only way of evaluation of protein activity. Depending on the nature of proteins and specific goals, we offer development of assays based on secretory responses, promoter activities and other cellular reactions.